TY - JOUR UR - http://stacks.iop.org/2399-1984/2/i=4/a=045005 T1 - Femtosecond photonic viral inactivation probed using solid-state nanopores A1 - Mina Nazari and Xiaoqing Li and Mohammad Amin Alibakhshi and Haojie Yang and Kathleen Souza and Christopher Gillespie and Suryaram Gummuluru and Mi K Hong and Björn M Reinhard and Kirill S Korolev and Lawrence D Ziegler and Qing Zhao and Meni Wanunu and Shyamsunder Erramilli Y1 - 2018 SP - 045005 JF - Nano Futures VL - 2 IS - 4 SN - 2399-1984 N2 - We report on detection of virus inactivation using femtosecond laser radiation by measuring the conductance of a solid state nanopore designed for detecting single particles. Conventional methods of assaying for viral inactivation based on plaque forming assays require 24–48 h for bacterial growth. Nanopore conductance measurements provide information on morphological changes at a single virion level. We show that analysis of a time series of nanopore conductance can quantify the detection of inactivation, requiring only a few minutes from collection to analysis. Morphological changes were verified by dynamic light scattering. Statistical analysis maximizing the information entropy provides a measure of the log reduction value. This work provides a rapid method for assaying viral inactivation with femtosecond lasers using solid-state nanopores. ER -